hcg production by elisa  (R&D Systems)

Journal: bioRxiv

Article Title: Transporter-Mediated Uptake of Microcystin-LR in Human Trophoblasts: Regulation By Oxygen Concentration and Cell Fusion

doi: 10.64898/2026.03.22.713491

Figure Lengend Snippet: BeWo cells were treated with either 0 or 20 μM forskolin (FOR) for 48 hours to stimulate fusion. (A) Intracellular cAMP and HCG secretion were quantified by ELISA. (B) Fluorescein uptake (0 or 1 μM) into control (CON) and forskolin (FOR)-treated BeWo cells was measured over 10 minutes (ex/em: 494/515 nm) and compared to a standard curve. (C) MC-LR uptake (0 or 1 μM) into control (CON) and forskolin (FOR)-treated BeWo was quantified after 1 hour. (D) Protein expression of OATP isoforms (1A2, 2B1, and 4A1) and protein phosphatases (PP1A and PP2A) were measured in control (CON) and forskolin (FOR)-treated BeWo cells. Data represented as mean ± SD (n=3-6) Statistical significance was assessed using an unpaired t-test, *p<0.05 compared to control.

Article Snippet: Cells were then treated with forskolin (vehicle or 20 μM) for 48 hours, after which media was collected to measure hCG production by ELISA (R&D systems, Cat #DY9034-05) as a marker for syncytialization.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Expressing

Journal: Cancer Cell International

Article Title: Dual-biomarkers encoding tumor-activatable minicircles with scaffold/matrix attachment region motif for sensitive and sustained blood and urine-based cancer detection

doi: 10.1186/s12935-026-04241-2

Figure Lengend Snippet: hCG and Gluc are reliable biomarkers with minimal background signals. HeLa cells were either untransfected or transfected with EF1 promoter-driven plasmids (PPs) encoding hCG together with eGFP (PP-pEF1-eGFP-M18) or Gluc (PP-pEF1-Gluc-M18). ( A ) Secreted hCG levels in culture supernatants were quantified by ELISA. Both transfected groups exhibited significantly higher hCG levels compared with untransfected controls. ( B ) Gluc level in supernatants was measured by a luminometer. Robust signals were detected only in the Gluc-transfected group. ( C ) Intracellular Gluc expression was visualized by bioluminescence imaging (BLI), again restricted to Gluc-transfected cells. Together, these results demonstrate that Gluc functions as a sensitive biomarker in both liquid assays and imaging applications. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18

Article Snippet: The human chorionic gonadotropin beta DuoSet ELISA kit (Cat# DY9034; R&D Systems) was used to quantify hCG levels in cell culture supernatants, urine, and plasma, in accordance with the manufacturer’s instructions.

Techniques: Transfection, Enzyme-linked Immunosorbent Assay, Expressing, Imaging, Biomarker Discovery, Control

Journal: Cancer Cell International

Article Title: Dual-biomarkers encoding tumor-activatable minicircles with scaffold/matrix attachment region motif for sensitive and sustained blood and urine-based cancer detection

doi: 10.1186/s12935-026-04241-2

Figure Lengend Snippet: Transfection with MC-pSURV-M18 restores biomarker expression reduced by promoter swapping. HeLa cells were transfected with PP-pEF1-M18, PP-pSURV-M18, or MC-pSURV-M18 to evaluate the effects of promoter swapping and minicircle constructs on biomarker expression. ( A ) hCG ELISA showed that replacing the constitutive EF1 promoter with the tumor-specific SURVIVIN promoter substantially reduced hCG expression. However, MC-pSURV-M18 transfection restored hCG expression to levels comparable to PP-pEF1-M18. ( B ) Gluc assay and ( C ) Gluc imaging demonstrated similar trends: promoter swapping decreased expression, whereas the corresponding minicircle construct rescued the loss. ᵃP < 0.001 vs. untransfected control; ᵇP < 0.001 vs. PP-pEF1-M18; ᶜP < 0.001 vs. PP-pSURV-M18

Article Snippet: The human chorionic gonadotropin beta DuoSet ELISA kit (Cat# DY9034; R&D Systems) was used to quantify hCG levels in cell culture supernatants, urine, and plasma, in accordance with the manufacturer’s instructions.

Techniques: Transfection, Biomarker Discovery, Expressing, Construct, Enzyme-linked Immunosorbent Assay, Imaging, Control

Journal: Cancer Cell International

Article Title: Dual-biomarkers encoding tumor-activatable minicircles with scaffold/matrix attachment region motif for sensitive and sustained blood and urine-based cancer detection

doi: 10.1186/s12935-026-04241-2

Figure Lengend Snippet: Tumor-activatable MCs encoding hCG and Gluc distinguish tumor-bearing mice from healthy controls. HeLa/Luc2-eGFP tumor-bearing mice were transfected in vivo with MC-pSURV-hCG-P2A-Gluc (MC) or MC-pSURV-hCG-P2A-Gluc-M18 (MC-M18). ( A ) Longitudinal Gluc imaging showed that MC-M18 sustained biomarker expression more effectively than MC. The hCG levels in ( B ) urine and ( C ) blood, measured by ELISA, were significantly elevated after transfection with either tumor-activatable MC, with or without the M18 S/MAR motif. ( D ) Urine and ( E ) blood Gluc levels showed greater variability and fluctuations; however, significant differences were detected between tumor-bearing mice transfected with MC (or MC-M18) and their respective healthy controls at specific time points. * p < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. * indicates comparing with the Control + MC-M18 group; # indicates comparing with the Control + MC group

Article Snippet: The human chorionic gonadotropin beta DuoSet ELISA kit (Cat# DY9034; R&D Systems) was used to quantify hCG levels in cell culture supernatants, urine, and plasma, in accordance with the manufacturer’s instructions.

Techniques: Transfection, In Vivo, Imaging, Biomarker Discovery, Expressing, Enzyme-linked Immunosorbent Assay, Control